rabbit αcav1 231  (Alomone Labs)

Journal: eNeuro

Article Title: Localization of TRP Channels in Healthy Oral Mucosa from Human Donors

doi: 10.1523/ENEURO.0328-21.2022

Figure Lengend Snippet: Antibodies used in this study

Article Snippet: Rabbit anti-TRPV3 , Alomone Labs , ACC-033 , ACC033AN02 , 1:100 , AB_2040261.

Techniques:

Journal: eNeuro

Article Title: Localization of TRP Channels in Healthy Oral Mucosa from Human Donors

doi: 10.1523/ENEURO.0328-21.2022

Figure Lengend Snippet: Antigen blocking shows specificity of TRP antibodies. A , TRPV3 immunoreactivity was identified in the foreskin epithelium. Antigen pretreatment blocked TRPV3 immunoreactivity ( N = 3 foreskins, paired t test p = 0.022). B , TRPV4 immunoreactivity was identified in the foreskin epithelium and was diminished with antigen pretreatment ( N = 7 foreskins, Wilcoxon matched pairs signed-rank test p = 0.016). Note, removal of outlier data point results in p = 0.02. C , TRPA1 immunoreactivity was identified primarily in the dermal compartment of foreskin tissue and was completely ablated with antigen pretreatment ( N = 4 foreskins or tongues, paired t test, p = 0.0016). D , TRPM8 immunoreactivity was localized primarily to the foreskin epithelium and portions of the dermal compartment. Antigen blocking greatly diminished immunoreactivity ( N = 3 foreskins, paired t test, N = 0.04). E , TRPV1 immunoreactivity was found primarily in intraepithelial nerve fibers of the foreskin. Antigen pretreatment completely abolished TRPV1 nerve fiber immunoreactivity but not the diffuse TRPV1 immunoreactivity of epithelial cells ( N = 3 foreskins, paired t test, N = 0.0004). All graphs show mean ± standard deviation.

Article Snippet: Rabbit anti-TRPV3 , Alomone Labs , ACC-033 , ACC033AN02 , 1:100 , AB_2040261.

Techniques: Blocking Assay, Standard Deviation

Journal: eNeuro

Article Title: Localization of TRP Channels in Healthy Oral Mucosa from Human Donors

doi: 10.1523/ENEURO.0328-21.2022

Figure Lengend Snippet: TRPV3 is expressed in basal layers of oral epithelium. Left column, Tuj1 anti-βIII tubulin (all afferent neurons). Second column, Anti-NFH antibody (myelinated neurons). Third column, Anti-TRPV3 antibody. Right column, Merge with TRP immunoreactivity in yellow, βIII immunoreactivity in cyan, NFH immunoreactivity in magenta. Dashed line indicates epithelia-lamina propria border. A , TRPV3 expression was found in basal epithelial layers of the hard palate as well as in the lamina propria. White arrows denote areas where Merkel cells typically concentrate . Note that Merkel cell afferents are visible with βIII tubulin staining in cyan. B , TRPV3 immunoreactivity was dispersed throughout fungiform epithelium. C , TRPV3 expression was found in basal epithelium of filiform papilla. A TRPV3 negative end bulb of Krause (arrow) is found within the lamina propria of the filiform papilla visualized with expression of βIII tubulin and NFH.

Article Snippet: Rabbit anti-TRPV3 , Alomone Labs , ACC-033 , ACC033AN02 , 1:100 , AB_2040261.

Techniques: Expressing, Staining